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From 2013.igem.org

5/16:
Group C:
Miniprep of overnight E. coli + pGAPzα C culture.
Restriction digest of miniprep samples 1-1, 2-1, 2-2 cut with either EcoRI or Bgl II.
Ran a get at 80 V for 90 mins with samples loaded in the following order: 1000 bp + ladder, 1-1 E, 1-1 B, 2-1 E, 2-1 B, 2-2 E, 2-2 B, 100 bp + ladder.
Made low salt LB broth
Made 200 mL overnight culture from 5/15 overnight culture 2-2.
Made 200 mL 1M sorbitol.
Autoclaved 1L di water for use transforming P. Pastoris.