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From 2013.igem.org

5/21:
Group A:
Made YPD broth.
Group B:
Miniprep of pGAPzα B.
Group C:
Miniprep of eight hour RFP culture from 5/20.
Digest of RFP with BglII.
Overnight culture of P. pastoris from overnight culture 5/20.
Digest of pGAPzα B and C with ECORI and XbaI.
Precipitate pGAPzα B and C DNA.
Ran gel confirming digestion of RFP.