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From 2013.igem.org

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Group C:
Digest 2μL linker B and C with EcoRI and NheI.

• Heat in activated for 20 minutes at 65°C.
  

Digest RFP samples 1-1, 2-1, 3-1 with BglII.

• Ran on gel at 70V for 90 minutes.
  

Digest pGAPzα B and C miniprep with EcoRI and XbaI.

• DNA precipitated resuspended in 50μL TE.
  
• Ran on gel at 70 V for 90 minutes.
  

Ran gel at 70V for 90 minutes with samples loading in the following order:

• 1kb + ladder, RFP 1-1, RFP 2-1, RFP 3-1, blank, pGAPzα B (3), blank, pGAPzα B (6), Blank, pGAPzα C (1), Blank, pGAPzα C (2).

Excised DNA from gel.