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From 2013.igem.org

2013-08-25

After returning from Hong Kong, we continued with main project. Because the construction of the main plasmid needed some more members, all the members responsible for experiments went to search on the main plasmid, our advisor was very toilsome helping us and he gave us many instructions

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We met many difficulties in this process. Firstly, we used the PCR device to amplify the parts we got, but we got nothing, the advisor suggested that maybe the primer was not proper, and it turned out to be like this. Then we did it again and got what we wanted. Eventually we attempted to linked the different parts we got and did the DNA gel electrophoresis, but the efficiency was so low that only few colored tapes were got on the gel. Under our advisor’s help, we made improvement and increase the time for PCR and change the method for PCR, and finally we got a satisfying result on the gel.

The procedure was very complicated and it took us a lot of time, and we stayed up late this night and the whole experiment was done 4:00am the next morning