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• Team:Heidelberg/Project/Tag-Optimization
  ...xidized by the Ox-domain, resulting in the blue pigment indigoidine. <i>E. coli</i> cells expressing the indigoidine synthetase appear blue when grown on a ...xidized by the Ox-domain, resulting in the blue pigment indigoidine. <i>E. coli</i> cells expressing the indigoidine synthetase appear blue when grown on a
  133 KB (19,473 words) - 02:56, 29 October 2013
• Team:Heidelberg/Templates/Materials list
  | ''E. coli'' DH10ß || New England Biolabs || C3019 | ''E. coli'' Top10 || invitrogen || C404010
  95 KB (12,744 words) - 07:35, 15 January 2014
• Team:Heidelberg/Project/Indigoidine
  <li> Endogenous PPTase of <em>E. coli</em> was proven sufficient for activation of the <em>P. lumninescens</em> d ...he endogenous 4'-Phoshopanthetheinyl-transferase (PPTase) entD from <em>E. coli</em> has been reported to exhibit low efficiency in activating heterologous
  60 KB (8,212 words) - 21:42, 17 October 2013
• Team:Heidelberg/Templates/MM week7
  ...phenicol acetyl transferase in insert: transform [[E. coli BAP1, competent|BAP1]] with 97 ng pLF03 (1 µl of miniPrep from 2013-06-04), plate on Amp + IPTG * evening: pick BAP1-pLF03 colonies, transfer to Cm + IPTG
  6 KB (843 words) - 03:29, 5 October 2013
• Team:Heidelberg/Templates/MM week6
  * inoculate 50 ml LB with BAP1, grow at 37°C until OD=0.57 * [[Preparing_glycerol_stocks|prepare BAP1 glycerol stocks]]
  4 KB (515 words) - 02:57, 5 October 2013
• Team:Heidelberg/Templates/Indigoidine week7
  We received the E. coli BAP1-strain which is commonly used for heterologuos expression of NRPS. It carri * co-transform [[E. coli BAP1, competent|BAP1]] with 84.5 ng pMM64 and 225 ng pMM65, plate on Amp + Kan + IPTG
  3 KB (465 words) - 00:41, 5 October 2013
• Team:Heidelberg/Templates/Indigoidine week6
  * transform [[E. coli BAP1, competent|BAP1]] with 169 ng pMM064, plate on LB+Amp+IPTG * plate contains BAP1-pMM064 colonies, but colorless
  2 KB (289 words) - 00:28, 5 October 2013
• Team:Heidelberg/Templates/Indigoidine week8
  ** [[E. coli TOP10, competent|TOP10]] * prepare primer for bpsA only plasmid for BAP1 validation
  2 KB (273 words) - 00:42, 5 October 2013