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  • Team:TU-Munich/Notebook/Labjournal
    Sequencing batch were prepared after manufacturer's protocol. (15 µl of plasmid DNA (50 - 100 ng) and 2 µl sequencing primer) * pSB1C3 plasmid with BBa_K801031 (PhyB 2 - 908 aa, RFC25): Colonies were picked from chlora
    804 KB (118,126 words) - 22:30, 22 October 2013
  • Team:XMU Software/html
    ...t"> <table id="i0s1i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textare ...t"> <table id="i0s2i6" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textare
    411 KB (51,065 words) - 05:18, 27 October 2013
  • Team:Freiburg/Project/toolkit
    ...g our plasmids, you will have to clone your target sequence into our crRNA plasmid (protocol see below). <td> RNAimer - plasmid (crRNA - plasmid) </td>
    87 KB (13,257 words) - 00:59, 29 October 2013
  • Team:SJTU-BioX-Shanghai/Notebook/Lab log
    ===1.Template Plasmid Amplification from Kit Plate=== ===2.Construct consititutional backbone plasmid===
    13 KB (1,826 words) - 01:11, 29 October 2013
  • Team:Newcastle/Notebook/calendar
    ...SB1C3] then PCR the insert before integrating it into the amyE integration plasmid (pSB<sub>Bs</sub>1C). We discovered that the Kanamycin resistance is an ''E *Groningen plasmid Mini-prep
    113 KB (17,146 words) - 14:46, 30 September 2013
  • Team:Goettingen/NoteBook w13
    ...39"><p class="c13-8 c13-3"><span>part6.4A C7 (102.6 ng/µl, 23.8.13, 3 µl plasmid + 1 µl HPLC water + 1 µl Primer 1:20)</span></p></td><td class="c13-21">< ...ot seen, but the band of re-ligated vectors was observed for re-ligand and plasmid control (&gt; 1 kb, as expected) and also for B C5→ since this band is no
    134 KB (22,952 words) - 21:14, 30 September 2013
  • Team:Paris Bettencourt/Notebook
    <TR><TD>Template Plasmid</TD><TD>0.25 ul</TD></TR> for Backbone (M13mp18 plasmid): 3ug<br>
    522 KB (75,300 words) - 03:51, 29 October 2013
  • Team:Goettingen/NoteBook w11
    ...omoter1<sub>rev</sub> , Promoter3<sub>rev</sub> or RBS<sub>rev</sub>, Mini Plasmid Prep of clones inoculated on 15.6.13 and preparation of some cryostocks, Te ... 6 – DarR</span><span class="c11-4">rev</span><span>&nbsp;C4 + VR (5 µl plasmid + 1 µl HPLC water + 1 µl iGEM_39 1:5)</span></p>
    105 KB (20,098 words) - 21:18, 30 September 2013
  • Team:Goettingen/NoteBook w12
    ...pan>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; </span><span>for all samples: 6 μl plasmid + 1 μl iGEM_38 1:5 or iGEM_39 1:5</span></p><p class="c12-8"><span class=" ...><span>Seq number</span></p></td><td class="c12-28"><p class="c12-7"><span>Plasmid + primer</span></p></td></tr><tr><td class="c12-50"><p class="c12-7"><span>
    165 KB (28,551 words) - 08:53, 17 October 2013
  • Team:HokkaidoU Japan/Shuffling Kit/Primer Designer
    ...369px 0}#plasmid-map #plasmid-9.plasmid-gray{background-position:-442px 0}#plasmid-map span{position:absolute;top:40px;left:-5px}.my-form{width:100%}.my-form .... Secondly, you hove to determine how many CDS are included in your target plasmid.</h3>
    15 KB (1,973 words) - 02:50, 29 October 2013
  • Team:XMU-China/wetlab journal
    Lane1:12D plasmid Lane2:12D plasmid, digestion of EcoRI,SpeI
    93 KB (15,921 words) - 03:09, 28 September 2013
  • Team:Heidelberg/Project/Tag-Optimization
    ...fficiency. Since this combinatorial approach requires the cloning of large plasmid libraries and hundreds of cotransformations, we established HiCT: High-thro ... host strains were previously transformed with a pSB3K3 derived expression plasmid coding for the PPTase Sfp from <i>B. subtilis</i>, which is commonly used i
    133 KB (19,473 words) - 02:56, 29 October 2013
  • Team:Freiburg/Notebook/standardisation
    ...only one band), new pSB1C3 is searched with RFC25 (–> Team Freiburg 2010 plasmid number 51).</<p> <h4>PCR of Fusion PCR1-7 and PCR over non-nickase Cas9 (plasmid 2004)</h4>
    128 KB (13,537 words) - 05:10, 28 October 2013
  • Team:Freiburg/Notebook/lab assembly biobricks
    ...only one band), new pSB1C3 is searched with RFC25 (–> Team Freiburg 2010 plasmid number 51).</<p> <h5>PCR of Fusion PCR1-7 and PCR over non-nickase Cas9 (plasmid 2004)</h5>
    132 KB (14,192 words) - 09:51, 1 October 2013
  • Team:SJTU-BioX-Shanghai/Notebook/Lab log/July
    ===Template Plasmid Amplification from Kit Plate=== ===Construct consititutional backbone plasmid===
    5 KB (725 words) - 03:17, 28 September 2013
  • Team:SJTU-BioX-Shanghai/Notebook/Lab log/August
    ===Construct dCas9, PcyA and Ho1 plasmid.=== ====PcyA-pRSF plasmid construction====
    5 KB (732 words) - 04:10, 28 September 2013
  • Team:OUC-China/Lab note
    2. Agarose electrophoresis: detect the plasmid PCR product.<br/> ...;"><font size="3px">Experiment:<br/>1. Agarose electrophoresis: detect the plasmid PCR product.<br/>
    81 KB (11,222 words) - 03:53, 28 September 2013
  • Team:Evry/Notebook/Test
    ...rains are competent or not, we also transformed our bacteria with a pSB1A3 plasmid (red colonies) and plated them on LB medium with ampicillin only.</p> <li> Plasmid 1K3
    113 KB (15,206 words) - 01:49, 5 October 2013
  • Team:Evry/Notebook
    ...rains are competent or not, we also transformed our bacteria with a pSB1A3 plasmid (red colonies) and plated them on LB medium with ampicillin only.</p> <li> Plasmid 1K3
    117 KB (16,033 words) - 02:34, 29 October 2013
  • Team:OU-Norman OK/Project/Notebook
    <div align="center" style="border:1px solid white"><b>PCR of i-GEM Plasmid Backbones Continued</b></div></br></br> <div align="center" style="border:1px solid white"><b>Adjusted PCR of iGEM Plasmid Backbones</b></div></br></br>
    189 KB (32,035 words) - 04:17, 28 September 2013

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