GFP under Arsenite Inducible Promoter ([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004])

Objective

Characterize the response of the arsenite sensitive promoter measuring GFP production upon different arsenite concentrations.

Methods

Several cultures carrying a plasmid that encodes GFP under this promoter([http://parts.igem.org/Part:BBa_K1106004 Bba_K1106004]) were grown overnight with different arsenite concentration (0, 10, 50, 500 and 1000 ppb). After sonication, fluorescence was measured at 395nm excitation peak and 515nm emission peak. Each measure was normalized by culture density (OD 600nm).

Results

As shown in the graphics below GFP production increases with the arsenite concentration.

Conclusion

The promoter is sensitive to different arsenite concentrations. Furthermore, we can discern among the critical arsenite concentrations according to the World Health Organisation.