Team:DTU-Denmark/Notebook/17 September 2013


17 September 2013

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Lab 223

Main purpose

The purpose of this experiment is to determine the long term characteristics of the growth of our Nir transformant anerobically. Specifically, we measure nitrous oxide production over a period of 10 hours.

Who was in the lab

Helen, Kasia, Ariadni


Following the protocol Bioreactor.


Nitrous Oxide measured in the bioreactor at the beginning and end of the experiment.

Time Replicate 1 Replicate 2 Replicate 3 Control: Distilled Water
14:00 1.84 mg/L 1.64 mg/L 1.60 mg/L N/A
22:00 1.43 mg/L 1.44 mg/L 1.50 mg/L 1.65 mg/L

At the end of the experiment the OD measurements were:

OD(end) sample A sample B sample C
- 0.0255 0.1590 0.0801

Colorimetric results


  • Measuring range 5-150 mg/L NH4-N
  • Measuring range 0.02-1 mg/L NO2-N

salt (mg/L) sample A/start sample A/end sample B/start sample B/end sample C/start sample C/end
ammonium 18 18 16 6 18 14
nitrite 0.93 0.91 0.92 1.72 0.9 0.9


There were several sources of error in this experiment. Most importantly, the experiment became aerobic in each chamber at 12:00, and the lab technician began sparging the reactors.

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