Lab 208

Main purpose

Run gel on yesterdays PCR.

Who was in the lab

Kristian

Procedure

Gel casting and loading

Results

• 1kb ladder
• araBAD
• araBAD
• araBAD
• Nir 1 min extension
• Nir 1 min extension
• pZA21::RFP no promoter
• pZA21::RFP no promoter
• pZA21::RFP no promoter
• Negative control
• Nir 1x
• Nir 1x
• Nir 5x
• Nir 5x
• 1kb ladder

Next line:

• 1kb ladder
• Nir 10x
• Nir 10x
• Nir 50x
• Nir 50x
• Nir 100x
• Nir 100x

• 1kb ladder
• Nir 500x
• Nir 500x
• Nir 2022
• Nir 2022
• Nir 2023
• Nir 2023
• Nir 2024
• Nir 2024
• Nir 2025
• Nir 2025

Conclusion

PCR is obviously not working too well for us right now. Tomorrow all reagents will be chanced and some of the PCR's will be run again.

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