208 lab

Main purposes today

New PCR with x7-polymerase.

Who was in the lab

Kristian

Procedure

Made PCRs on pZA21, GFP SF TAT, RFP all samples where made in duplicates.

• In tube 1+2, 5+6 where pZA21.
• In 9+10 GFP SF TAT
• In 3+4, 7+8 RFP

First program was 55°C annealing and 2:00 extension time with tube: 1+2, 3+4. Second program was 64°C annealing and 2:00 extension time with tube: 5+6, 7+8. Last program was 69°C annealing and 1:00 extension time with tube: 9+10.

Results

None of the PCRs worked.

Conclusion from today

New PCR programs need to be tested

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