Team:Groningen/Labwork/17 August 2013

Inne

For the plates of the motility assay only the 0.4% provided a probable result. The others were completely overgrown, the reason for this is very likely that the concentration of agar (0.2% and 0.1%) is to low for plates. The tests did show that delta cheY bacillus grows better, and more uniformly, then competent E.coli.

The hy-spank GFP e.coli plates grew colonies, 4 single colonies are picked and inoculated in the 37C shaker (overnight).

Performed a restriction digest of ms2 samples 1 till 3.
0.8% gel was loaded as following.

well 1	well 2	well 3	well 4	well 5	well 6	well 7	well 8	well 9	well 10
1kb DNA ruler	ms2 1	ms2 2	ms2 3	uncut plasmid ms2 1	1kb DNA ruler	ms2 1	ms2 2	ms2 3	uncut plasmid ms2 1

The gel was loaded in duplo because there was to much sample to fit in one well
the gel didn't show any bands except for the DNA ruler.