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Team:Groningen/Labwork/25 September 2013
From 2013.igem.org
Mirjam
Two colonies are chosen and inoculated in LB medium with Cm to higher the amount of the Pdes-cheY construct. Did a miniprep on the samples.Inoculations of ΔDesΔCheY and WT bacillus strains in minimal medium.
Did a gel purification of CheC up and CheC down. Did restriction analysis of CheC up, CheC down and Spec. Ligation of CheC. Transformation of CheC in the ΔDesΔCheY strain.
iGEM 2013 Groningen
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