Team:Groningen/Labwork/26 June 2013

From 2013.igem.org

Mirjam

Did a genomic DNA extraction of B. subtilis strain 168. Two tubes are obtained from this with a concentration of 66ng/µl and 67ng/µl.

The primers for our main subject, silk secretion, arrived. The primers are diluted to a concentration of 10mM.
To run a PCR, dNTPs are necessary in a concentration of 10mM, so this dilution is also made.

A PCR reaction mix is made for the four different signal sequences.

signal sequence Tm-5 size
FliZ 45°C 130
MotB 50°C 100-150
EstA 50°C 100-150
LytB 50°C 100-150

The PCR product is loaded on gel to examine if the PCR reaction succeeded.
picture

Claudio and Inne

Further discussion about the available options to create a heat-attracted bacteria.