Team:Groningen/Labwork/27 August 2013

Inne

Did a mini prep of the colonies with biobrick BBa_k1085006, samples s7,s8,s11,s12,s13,s14. 30 uL elution buffer was used instead of 50 uL. Determined the concentration of the samples via nanodrop, also nanodropped the samples Sander miniprepped.

Sample	Concentration
s3	75.6 ng/L
s4	93.3 ng/L
s9	87.9 ng/L
s10	79.4 ng/L
s7	91.6 ng/L
s8	82.8 ng/L
s11	94.8 ng/L
s12	86.6 ng/L
s13	105.6 ng/L
s14	104.5 ng/L

Inoculated the motility assay plate with wt, delta cheY and delta cheY/des. All strains will be grown in duplo overnight at 37C and 30C, time of inoculation: 18:20.

Sander

Did a mini prep of the colonies of samples s3,s4,s9,s10. Did a restriction digest of the samples and ran them over a 0,8% agarose gel. the samples were not present in the plasmids.

Sebas

Designed and ordered primers to turn around the hy_spank promoter to end up with our final (correct) backbone:
Found another backbone (pX), not biobrick compatible but with sites SpeI*SdaI (same overhang as XbaI*PstI).