Team:Groningen/Labwork/3 July 2013



Run a 0.8% agarose gel to examine the purified promoter, gel run at 100V for 22 minutes.

Mirjam and Sander

The gel examined that again dimerization of the primers happened. Therefore a new PCR run is made with the addition of DMSO. Turned out that the one with DMSO had better results. Did a gel purification (standard protocol).

Sander and Inne

Did a PCR for the silk template, in duplo. With the following protocol:
1 2 3 4 5 6
98 98 85 72 72 4
2:00 0:30 0:25 0:27 10:00 forever So two with DMSO and two without. NB: another change is that we used 5% DMSO this time Ran a gel which had more bigger bands them the previous pcr. The samples without DMSO were clearer than those with DMSO.