Team:Heidelberg/Templates/Del week14 G

From 2013.igem.org

29-07-2013

Restriction digest of fragment FS_08 to FS_23; 6.5 kb; 23-07-2013 with ClaI

Test restriction digest (29-07); run at 100 V, 0.8 % gel (TAE)

Incubation at 37°C for about 3 hours

what µl
FS_08 to FS_23 (2) (23-07-2013) 15
ClaI 1
Buffer CutSmart 2
dd H2O 2
Expected fragment lengths [bp] 3291, 1936, 1303

Results:

  • Restriction digest of DelG (FS_08 to FS_23) was successful all predicted bands are clearly visible
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