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From 2013.igem.org

Indigoidine Production - bpsA

We performed PCR screenings of the pMM64 (bpsA) and pMM65 (svp) plasmid for validation.

The PCR screenings showed that the pMM-plasmids are correct. We designed primers to assemble our first pSB1C3 derived plasmid pKH1, which carries both the bpsA and the svp gene with the lacI-Promoter BBa_R0010 and the ribosome binding site BBa_B0034 before both the bpsA and the svp gene, respectively..