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From 2013.igem.org

Contents 1 Cloning Techniques/Strategies (Dominik) 2 Discussion on Experimental Procedures 3 NRPS Library - with Discussion 4 Agenda for next meeting (29.04.2013) 5 Ressource Collection 6 further planning

Cloning Techniques/Strategies (Dominik)

• BBa (BioBrick Standard)
• Golden Gate
• Gibson Assambly

Discussion on Experimental Procedures

• Transformations
• Cosmids
• BACs

NRPS Library - with Discussion

• main ideas
• swaping of domains done already
• Q: Biology behind the System? (short presentation by Ilia on monday?)

Agenda for next meeting (29.04.2013)

• presentation of NRPS (library) (Ilia)
• presentation about methodology (BAC, PAC, Cosmide, Gibson Assambly (Fanny, Flo, Hanna, Joshua, Nicos)
• schedule (Hanna, Julia) ?

Ressource Collection

• Vector Design
  • [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI]
  • [http://www.geneious.com/ Geneious]

• Gibson Assembly
  • NEB Discripton & Kit
  • Reference:

1. Gibson, D.G. et al. (2009) Nature Methods, 343–345.
2. Gibson, D.G. et al. (2010) Nature Methods, 901–903.

• Reserach on NRPS
  • [http://www.uni-marburg.de/fb15/ag-marahiel/research AG Marahiel, Marburg] --> Niels
  • [http://www.mpimf-heidelberg.mpg.de/gruppen/cytochrome AG Cryle, MPI Heidelberg] --> Konrad

further planning

• Sat. 4. May: primer Design meeting ("Giant Inserts in E. coli")