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From 2013.igem.org

Contents 1 iGem42 - input on Design: 2 lab update 3 organization 4 Agenda for next meeting(02.07.2013)

iGem42 - input on Design:

1. no greek
2. round edges
3. one uniform lettering
4. design: where am I?
5. dark blue, rest modern

=> 3 different designs until tuesday

lab update

1. general
   • polymerase dosn't work as expected (Q5 in contrary works)
   • Phusion Flash from advisors works also
   • CyclerProblems: Gradienten-PCR doesn't work
2. indigoidine
   • streptomyces strain arrived => monday culture the streptomyces
   • plasmid containing sc-indc is ordered
   • primer monday to Dominik and then order
3. DelH
   • colonies after electroporesis had no integrated DelH
   • amplification of DelH fragments again didn't work - only with Phusion Flash (1b & 2)
   • Backbone is not amplified (pSB6A1-AraC-lacZ)
4. Del-Rest
   • Primers are ordered
   • since wednesday: Del-rest amplify from the genome (Flo & Nils)

organization

1. Google-Kalendar for all
2. responsibility of ordering stuff: Sophie
3. create Milestones & lab managment on the wiki: Sophie
4. tyrocidine has to be started = parts for sending in (check-liste)
5. wiki team (Ralf, Flo und Sophie) check the list for gold medal!

Agenda for next meeting(02.07.2013)

1. Milestones - Groups make the Milestones for their group erstellen diese mit Gruppe
2. timeschedule ?who, when?
3. iGEM42
4. Sponsoring update