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From 2013.igem.org

Contents 1 Sponsoring 2 Wiki-Design & T-shirts 3 Lab update 4 NRPS-Software 5 Art Cooperation

Sponsoring

• Böhringer Ingelheim sponsors us with 5.000 €
• a Gold Smith from Heidelberg donates us 50 €

Wiki-Design & T-shirts

• we decided to stich our logo on the shirts
• color: navy
• golden border around the logo with blue background

Lab update

Indigiodine

• we have 3 constructs which work
• now we want to find out which T-domains are the best to use for the NRPS library
• we are going to clone indigoidine into igem-standard and characterize it

Tyrocidine

• a few fragments don't have sufficient concentration --> optimization
• amplification of some fragments didn't work
• first gibson assembly will be done this week
• talked to different people because of using their mass-spectrometry

DelH

• changed the backbone
• managed to create constructs, did the electroporation
• there were no colonies growing on the plates

DelRest

• were able to amplify all parts
• started to validate fragments
• start gibson as soon as we checked fragments

NRPS-Software

• Database is more or less implemented
• main programm is able to most of what it is supposed to
• Webinterface has been started but doesn't comprise all features
• connection Pfam works

Art Cooperation

• maybe involving artists into our meeting
• talk to them about our projects --> maybe they like some parts of our projects and we can collaborate
• we could give them a insight into synthetic biology
• we can think about offering some of the artists to stay with us during the period of the art project