Team:Heidelberg/Templates/MM week14

From 2013.igem.org

2013-07-29

  • inoculate 10 ml 2xYT+Amp with TOP10-pKD46, grow at 30°C

2013-07-31

miniPrep of pKD46. Lane 1: NEB 2-log (5 µl), lane 2: 1 µl of miniPrep
  • miniPrep of pKD46 -> ca. 10 ng/µl

2013-08-03

  • transform BAP1 with 5µl of pKD46 miniPrep form 2013-07-31, plate on Amp, grow at 30°C
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