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From 2013.igem.org

2013-07-29

• make miniPrep -> very low yield (1-2 ng/µl)
• load 1µl of 97ng/µl pLF03 miniPrep from 2013-06-04 on gel -> ca. 20 ng/µl
• inoculate 50 ml LB+Amp with TOP10-pLF03, grow at 37°C

2013-07-30

• make midiPrep -> 1352 ng/µl in 66.5 µl
• medium was contaminated (also check with Fanny) -> low yields of previous miniPreps

2013-07-31

• send 20 µl of 1:15 dilution of midiPrep to sequencing with primers IK07, IK36, IK37: File:Heidelberg PLF03 seq 2013-07-31.zip
• perform multiple sequence alignment against assumed pLF03 sequence: IK07, IK36, IK37
• deletion at the end of accA2 -> primer can not bind