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From 2013.igem.org

Contents 1 RFC10 Standardization of Tyc Modules 1.1 Primer Design 1.2 Amplification of Tyc A 1.3 Amplification of Tyc B1 1.3.1 A

RFC10 Standardization of Tyc Modules

Primer Design

Amplification of Tyc A

TycA	µl
Bacillus	1
AT01	2
AT02	2
Q5 2x Master mix	10
ddH20	5

Cycles	temperature [°C]	Time [min:s]
1	98	2:00
35	98	0:10
	59	0:30
	72	1:30
1	72	2:00
1	10	inf

Result There was a band at the right length but we were not able to cut.

Amplification of Tyc B1

A

TycB1	µl
Bacillus	1
AT03	2
AT04	2
Q5 2x Master mix	10
ddH20	5

Cycles	temperature [°C]	Time [min:s]
1	98	2:00
35	98	0:10
	56	0:30
	72	1:30
1	72	2:00
1	10	inf

Result no band in gel, empty lane; optimization