Team:HokkaidoU Japan/Shuffling Kit/Primer Designer

From 2013.igem.org

Maestro E. coli

Shuffling Kit

Primer Designer for Maestro

1. First, you have to decide whether or not you conform to our default overhang set.

2. Secondly, you hove to determine how many CDS are included in your target plasmid.

I have CDS(s) included.

3. Then, please select the region to where your fragment correspond in target plasmid.

1
2
3
4
5
6
7
8
9

4. Okey, now we are ready to go. Enter your fragment's sequence, and press "Calculate"!

2. Please enter overhangs and fragment's sequence, and press "Calculate"





Forward

sequence
tm

Reverse

sequence
tm

5. Now, repeat previous step for remaining fragments included in the target plasmid.