Team:Paris Bettencourt/Notebook/Drug Screening/Saturday 21st September.html



Saturday 21st September

Weekend Rush

Gibson assembly of pSB9S1, pSB10K1, and pSB3C7.

Digestion of sirA, fprA, fdxA and linearized backbone with XbaI and SpeI

Digestion of Linearized pSB1C3 with XbaI, SpeI, and DpnI

Ligation of sirA, fprA, and fdxA with pSB1C3

Heat Shock transformation of pSB1C3 sirA, pSB1C3 fprA and pSB1C3 fdxA into NEBTurbo

Heat Shock transformation of pSB9S1, pSB10K1 and pSB3C7 into NEBTurbo