Team:Paris Bettencourt/Notebook/Drug Screening/Tuesday 17th September.html


Drug Screening

Tuesday 17th September

Transforming gBlock FdxA and Biobricking FprA and SirA

Ligations were transformed into E. coli NEBTurbo Chemically Competent Cells by Heat Shock transformation. Cells were recovered for 1.5 hours before being plated. pSB1c3 fprA and pSB1c3 SirA were plated onto Chloramphenicol plates (34ug/ul) and pET FprA FdxA were plated onto Ampicillin (50ug/ul) plates.