Team:Paris Bettencourt/Notebook/Drug Screening/Tuesday 2nd July.html

From 2013.igem.org

Drug Screening

Tuesday2nd July

MiniPrep: Plasmids pD004, pD005 and pD006 were extracted from NEBturbo vectors

Plasmid Extraction
Plasmids pD004 (pCDF.ew12 zmFNR Spectinomycin), pD005 (pACYC.ew13 soFD, zmSIR Chloramphenicol), pD006 (pACYC.ew17 zmSIR Chloramphenicol) were extracted from NEBTurbo cells using a Thermo Scientific GeneJet Plasmid mini prep kit as described in protocol (). Lacking Resuspention solution we used some from a different mini prep upstairs. Plasmids were eluted in 100ul of nanopure water and frozen at -20ºC. More notes here tra la lalal

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