Team:Paris Bettencourt/Notebook/Phage Sensor/Thursday 1st August.html

Phage Sensor

ASDF

Thursday 1^st August

PCR (gradient + usual) of SPCR1, SPCR7, SPCR8, SPCR10, SPCR9, SPCR4

Reagent	Volume
	1x
Nuclease-free water	37.25 µL
5x Phusion HF Buffer	10 µL
10 mM dNTPs	1 µL
Forward Primer (10 µM)	0.5 µL
Reverse Primer (10 µM)	0.5 µL
Template Plasmid	0.25 µL
Phusion DNA Polymerase	0.5 µL
Total Volume	50 µL

Gradient:
Pos 1: 40°
Pos 2: 40,2°
Pos 3: 41,3°
Pos 4: 43,1°
Pos 5: 45,4°
Pos 6: 48°
Pos 7: 50,7°
Pos 8: 53,5°
Pos 9: 56,0°
Pos 10: 58,1°
Pos 11: 59,8°C
(Pos 12: 60,6°)

Usual PCR: 52°