Team:Paris Bettencourt/Notebook/Phage Sensor/Thursday 24th October.html

Detect

ASDF

Thursday 24^th October

Digest phagemid E/P
Purify it

Ligation:
1. SPCR3 E/P + phagemid E/P
2. SPCR3 E/P + SPCR5 P/X+ phagemid E/X
2. SPCR3 E/P + SPCR2 P/X+ phagemid E/X

trafo Ligations

electrocmpetent cells parental+sSP009+pS002’
trafo:
1. parental+pS002
2. parental+pS002’
3. parental+pS006*
4. parental+pS002’+pS006*
5. sSP009 pS002’ + pS006*

trafo helper into NEB

liquid cultures: sSP009, pRECA strains Ariel, sSP008+pS002, sSP008+pS002’