Team:Paris Bettencourt/Notebook/Phage Sensor/Wednesday 17th July.html


Phage Sensor

Wednesday 17th of July

PCR purification

We are purifying the colony PCR from yesterday to send it away for sequencing

Add 1.1 volume and Binding Buffer
Transfer up to 800 µL. Centrifuge for 30-60 seconds. Descend the flow through
Add 700 µL of washed Buffer. Centrifuge for 30-60 seconds
+ 1 min centrifuge to completely remove any residual wash buffer
Add 50 µL of Elution Buffer to the center of the Gene JT purification column membrane and centrifuge for 1 minute
Discard the Gene JET purification column and store the purified DNA at -20°C.