Team:Paris Bettencourt/Notebook/TB-ception/Monday 2nd September.html

From 2013.igem.org

TB-ception

Monday 2nd September


Transformed BL21 + pET21b with pColA + J23102/104 (electroporation and heat shock), transformed BL21 + pET21b with pColA + J23102/104 and with the LLO plasmid. The PCR for the pET21b worked and we've sent it for sequencing; the PCR for the LLO plasmid didn't work :(