Team:Paris Bettencourt/Notebook/TB-ception/Thursday 29th August.html

From 2013.igem.org

TB-ception

Thursday 29th August


We did a phagocytosis assay for our macrophages. First we passaged our cells and we determined their number (colored them with trypan blue and counted them): 2.68* 106, 3.72* 106 and 1.32* 106. To the first one we added 40, to the second 30 and to the last one 90 ┬Ál of NEB + pSB1A3 cell suspension. After 1 h we made this awesom picture:



Cloning from yesterday didn't work, so we repeated everything with longer digestion time for the backbone.