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From 2013.igem.org

Redsign of Primers
Design primers to PCR KanR out of Duet and LacZalpha out of pUC18 to then clone them into the DUET containing Chloramphenicol.
Geneious files in the dropbox: LacZ primers, KanR primers.

Liquid Cultures (for Miniprep)
7ml LB with proper antibiotics adding:

• NEB with pUC18 (AmpR)
• sT002 : MG1655 pCOLADuet-1 (KanR)
• sT003 : MG1655 pACYCDuet-1 (ChlaR)

Cultivate over night in the 37°C shaker

Check: GFP expressed as expected in ELS-41
Lauching O/N of ELS-41 and ELS-13 with Amp and Kan, respectively.

Lauching O/N of MGZ1 (Glycerol from lab upstairs) with Spec (500X)