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From 2013.igem.org

Labbook |	Protocols |	Materials |

Contents 1 Ampicillin 1.1 About 1.2 Stock 1.3 Stability 1.4 References

Ampicillin

About

Ampcillin is a beta-lactam antibiotic that acts as a competitve inhibitor of transpeptidase an enzyme required for cell wall synthesis. Ampicillin works on most gram-positive and some gram-negative bacteria.

Stock

1. Mix:
   • 4 g ampicillin (100 mg/mL)
   • ad. 40 mL dist. water
2. sterile filtration
3. aliquot in 1 mL stocks and store at -20°C

Stability

• Ampicillin is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
• Culture plates can be stored for about 2 month at 4°C (as a batch in a bag or individually sealed with Parafilm)

References

• http://en.wikipedia.org/wiki/Ampicillin