Team:TU Darmstadt/materials/Chloramphenicol
From 2013.igem.org
Labbook | | Protocols | | Materials | |
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Contents |
Chloramphenicol
About
Chloramphenicol is a bacteriostatic antibiotic that specifically binds resudies in the 50S ribosomal subunit, preventing peptide bond formation.
Stock
- Mix:
- 1 g chloramphenicol (25 mg/mL)
- ad. 40 mL ethanol
- aliqout in 1 mL stocks and store at -20°C
Stability
- Chloramphenicol is not autoclavable, it has to be added before pouring plates (keep in mind that the agar is not too hot when adding it)
References
http://en.wikipedia.org/wiki/Chloramphenicol