"

From 2013.igem.org

Labbook |	Protocols |	Materials |

DYT

Ingredients for 1.7- DYT medium

For 500 mL

• 2.5 g NaCl
• 8.0 g tryptone
• 5.0 g yeast extract

For 100 mL

• 0.5 g NaCl
• 1.6 g tryptone
• 1.0 g yeast extract

Continue to:

• mix in ddH₂O
• add 5 M NaOH to adjust the pH at 7.0 (few drops will be enough)
• aliquot: add 3 ml to 15 ml tubes (about two thumbs high) cap loosely
• autoclave in a rack in a tub with at least 4 cm of water
• store at 4°C