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From 2013.igem.org

Labbook |	Protocols |	Materials |

Contents 1 Phusion 1.1 About 1.2 Working conditions 1.3 References

Phusion

About

Phusion is a Pyrococcus Pfu-like DNA polymerase with a dobule-strand DNA binding domain, resulting in an enhanced processivity and higher activity than other available DNA polymerases like taq and creates blunt ends.

Working conditions

• 98°C denaturation temperature
• ~30 s/kb extension velocity (at 72°C)
• (T_M-3)°C=T_A

References

• http://www.neb.com/products/m0530-phusion-high-fidelity-dna-polymerase