Team:Tuebingen/Notebook/Protocols/pcr

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PCR

Reagents

39.5 µL Aqua dest.
2.5 µL dNTPs (200 µM each)
1 µL DNA (c = 100 ng/µL)
5.0 µL Taq-Buffer
0.5 µL Forward primer
0.5 µL Reverse primer
1 µL Taq/Pfu polymerases (9+1)

 

Procedure

Mix all reagents in the given order (Important: add polymerases last!) and transfer in one 0.2 mL Eppendorf-tube ("PCR-tube"). Run cycler according to your requirements.

This protocol yields exactly one single reaction mixture! Scale linearly for multiple reaction mixtures and keep mixture on ice or even better at -20 °C for best results.

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