Team:Tuebingen/Notebook/Protocols/sob-medium
From 2013.igem.org
![](https://static.igem.org/mediawiki/2013/5/50/IGEM_basic_Logo_white_stylized_small.png)
![](https://static.igem.org/mediawiki/2013/8/86/IGEM_basic_Logo_stylized_small.png)
SOB Medium
Reagents
20.0 g | Trypton |
5.0 g | Yeast extract |
0.5 g | NaCl |
10 mL | KCl (c = 250 mM) |
Procedure
In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).
Back to Protocols