Team:Tuebingen/Notebook/Protocols/sob-medium

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SOB Medium

Reagents

20.0 g Trypton
5.0 g Yeast extract
0.5 g NaCl
10 mL KCl (c = 250 mM)

 

Procedure

In an autoclavable flask, dissolve all reagents in 1000 mL Aqua dest., adjust pH to 7.0 (with NaOH). Sterilize by autoclaving. After autoclaving add 5 mL MgCL2 (c = 2 M).

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