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CRISPRi Conjugation
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General Protocals
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Basic Protocols for cloning and working with
E. coli
Colony PCR
Competent Cells Using Electroporation
E. coli
Transformation
Gel Extraction
Gels
Gibson Assembly
In-Fusion Cloning
Making Glycerol Stocks
Mini Prep
PCR Phusion
PCR Purification
PCR Sewing
Restriction Digest
Seamless Cloning & Assembly