"

From 2013.igem.org

{ "date" : "2013-05-30", "author" : "viola-fabio-michele", "title" : "Transformations and screening parts from 2012 plates", "content" : "We prepared some agar plates with tetracyclin (10ug/ml) and some Luria Broth. Then we extracted, transformed in competent cells and plated the following parts :

part	plasmid	plate	colonies
Bba_J45119	pSB1AT3	amp, tet 10µg/ml, tet 50µg/ml	only in amp plate
Bba_J45320	pSB1AT3	amp, tet 10µg/ml, tet 50µg/ml	only in amp and tet 10µg/ml
Bba_J45 700	pSB1AK3	kan	yes

We also screened the parts purified by Fabio and Gire :K1,K2,K3 = BBa_J45319A1,A2=BBa_J45004", "tags" : "PchB-PchBA-BSMT1" }