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From 2013.igem.org

{ "date" : "2013-06-14", "author" : "thomas", "title" : "EFE extraction and transformation Test!", "content" : "Since we received the Ethylen Forming Enzyme gene from Genescript company, we proceeded with the extraction and the transformation test.We resuspended the 4 ug of DNA in 40 ul of sterilized water (obtaining a 100 ng/ul stock solution). After that, we transformed 200 ul of NEB10 competent cells with 1ul of EFE DNA.Finally we plated them in two 50 ug/ml Amp LB-Agar Petri dishes.Results:

As you can see from the image, we obtained many colonies. We then inculated x5 colonies in 5ml LB with Ampicillin.", "tags" : "EFE" }