"

From 2013.igem.org

{ "date" : "2013-06-17", "author" : "fabio-bruno", "title" : "bacillus subtilis promoters (part2)!!", "content" : " with some tips we selected 2 colonies for each promoter and then put them in LB ( 10 ml) for an overnight incubation (under shake). In the meanwhile we received a new e.coli strain ( NEB5a) and made an inoculum overnight. ", "tags" : "Pveg-PliaG-PlepA" }