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From 2013.igem.org

{ "date" : "2013-06-17", "author" : "thomas", "title" : "Cloning of EFE in pSB1C3", "content" : "I began the week doing some minipreps (x5 of EFE and x5 of pSB1C3+RFP) following this protocol.

I then digested the linearized pSB1C3 (500 ng, kindly offered by Caterina), pSB1C3 + RFP (500 ng) and EFE (1000 ng) with EcorI and PstI following the 2A assembly protocol.After that, I proceeded with the ligation and transformation in NEB10B cells. ", "tags" : "EFE" }