{ "date" : "2013-06-20", "author" : "emil", "title" : "Linearization of psB1C3 (Failed)", "content" : "I have re-tried to linearize through a PCR the backbone PsB1C3 that we have previously purified and quntified.
I have tried to amplify the sample of 117.3 ng/µl, 105.7 ng/µl and one of Girelli's sample(184.1 ng/µl)following the Phusion PCR protocol and the universal primers.
No one of this PCRs has succeded!

Linearization attempt 2
width=\"450px\" />
", "tags" : "pSB1C3" }