Team:UNITN-Trento/Notebook/Labposts/06/62
From 2013.igem.org
{ "date" : "2013-06-27", "author" : "emil", "title" : "Transformation of 2 GFPs and inocula of B. subtilis backbones", "content" : "Emil transformed two construct of the ancient Igem competition: both consist in a RBS with the GFP(E0040) and 2 terminators(B0010)(B0012) and are contained in bSB1A2
GFP
Emil followed the transformation protocol loading 150 ng of each sample.Then Emil plated the product on an Ampicillin plate.ID | Quantification | RBS strength |
---|---|---|
BBa_E0240 | 108.5 ng/µl | medium |
BBa_E0840 | 129 ng/µl | strong |
Moreover Emil did the inocula (in LB + Ampicillin) of two backbones(BBa_823024 and BBa_823026) from the plates of Fabio and Bruno(2 inocula for each sample).", "tags" : "BBa_823024-BBa_823026-BBa_E0240-BBa_E0840-B. subtilis" }