"

From 2013.igem.org

{"date":"2013-07-02","author":"viola","title":"EFE+B0015","content":"today i digested B015 in pSB1C3 with the enzymes E and X and EFE in pUC57 with E and S. I started a short cloning protocol with this ligation quantities:

LIGATION	CTRL	1:1	1:2	1:4
BUFFER T4	2 µl	2 µl	2 µl	2 µl
PLASMID (B0015)	2.5 µl	2.5 µl	2.5 µl	2.5 µl
INSEERT (EFE)	0 µl	1.28 µl	2.56 µl	5.12 µl
LIGASE T4	1 µl	1 µl	1 µl	1 µl
H2O	14.5 µl	13.2 µl	11.9 µl	10.6 µl

","tags":"EFE"}