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From 2013.igem.org

{"date" : "2013-07-19","author" : "emil-viola","title" : "The incompetent Bacillus subtilis saga OD(io)","content" : "We have tied to grow B.subtilis in the minimal medium (Cambridge protocol and Mansy's friend's protocol) with no evidence of growth, in the previous days we have observed that B. subtilis grow in LB so we have tried to transform B. subtilis in LB following the LMU munich protocol modified.We transformed 2 groups of cells with the construct K823026+GFP but we mistaken the right Kanamicin concentration of the stock solution(10mg/ml)so no the following day ther were no colonies on the plates.","tags" : "Bacillus subtilis"}