"

From 2013.igem.org

{"date" : "2013-07-22","author" : "emil","title" : "The incompetent Bacillus subtilis saga: The mistaken antibiotic ","content" : "Unfortunately the previous attempt to transform failed maybe due to the mistaken concentration of antibiotic so I restarted generating a stock solution of Kanamicin(10mg/ml).I did an inocula in LB of Bacillus overnight from the stock solution with 50% of glicerol.","tags" : "Bacillus subtilis"}