Team:UNITN-Trento/Notebook/Labposts/07/58
From 2013.igem.org
{"date" : "2013-07-24","author" : "thomas","title" : "Screening of AraC-pBAD + EFE + Venus","content" : "This is the part five of this experiment. I proceeded with a miniprep purification of the previously prepared inocula and a screening digestion in order to verify the construct.
Quantification Results
Sample | Concentration |
---|---|
A from ligation 1:1 | 852.6 ng/ul |
B from ligation 1:1 | 1123 ng/ul |
C from ligation 1:4 | 685.8 ng/ul |
D from ligation 1:4 | 2128.3 ng/ul |
E from ligation 1:1 | 1760 ng/ul |
A from ligation 1:1 | 852.6 ng/ul |
As you can see from the gel image, only one sample seems to confirm our fusion protein and will be sent for sequencing. Lane 4: insert 3024 bp, vector 2070 bp. Kapa universal ladder was adopted.","tags" : "EFE-Venus"}